Abstract
Several amide constituents (piperlonguminine and retrofractamides A, B, and C) from the fruit of Piper chaba promoted adipogenesis of 3T3-L1 cells. Among them, retrofractamide A was the most active and significantly increased the amount of adiponectin released into the medium and the uptake of 2-deoxyglucose into the cells. Retrofractamide A also increased mRNA levels of adiponectin, peroxisome proliferator-activated receptor gamma2 (PPARgamma2), glucose transporter 4 (GLUT4), and insulin receptor substrate 1 (IRS-1), but did not act as a PPARgamma agonist different from troglitazone.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3-L1 Cells
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Adaptor Proteins, Signal Transducing / drug effects
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Adipogenesis / drug effects*
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Adiponectin / metabolism
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Amides / chemistry
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Amides / isolation & purification*
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Amides / pharmacology*
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Animals
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Benzodioxoles / chemistry
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Benzodioxoles / isolation & purification*
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Benzodioxoles / pharmacology*
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Dioxolanes / chemistry
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Dioxolanes / isolation & purification
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Dioxolanes / pharmacology*
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Glucose Transporter Type 4 / drug effects
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Insulin Receptor Substrate Proteins
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Mice
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Peroxisome Proliferator-Activated Receptors / drug effects
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Piper nigrum / chemistry*
Substances
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Adaptor Proteins, Signal Transducing
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Adiponectin
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Amides
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Benzodioxoles
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Dioxolanes
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Glucose Transporter Type 4
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Insulin Receptor Substrate Proteins
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Irs1 protein, mouse
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Peroxisome Proliferator-Activated Receptors
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retrofractamide A
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piperlongumine